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aavpro  (TaKaRa)


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    Structured Review

    TaKaRa aavpro
    Aavpro, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aavpro+concentrator/AAVpro+Concentrator/us12606845-220-14-20
    Average 93 stars, based on 14 article reviews
    aavpro - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Purification:

    Article Title: 2020 ASGCT Annual Meeting Abstracts
    Article Snippet: .. The crude lysate was further purified by using a part of components included in AAVpro® concentrator (Takara Bio) and AAVpro® purification kit (Takara Bio). .. The obtained viral genomic copy number was determined by the quantitative PCR using AAVpro® Titration Kit (for Real Time PCR) Ver.2 (Takara Bio).

    Article Title: Evasion of APOBEC1-mediated Intrinsic Immunity by a Herpesvirus Uracil DNA Glycosylase Is a Determinant of Viral Encephalitis
    Article Snippet: At 10 days post-transfection, the culture supernatant was centrifuged at 10,000 ×g for 15 min at 4°C and filtrated through a 0.22-μm filter (Thermo Fisher Scientific). .. Then, recombinant AAVs were purified using an AAVpro Concentrator (TaKaRa) according to the manufacturer’s instructions, and designated AAV- ZsGreen and AAV-UGI. .. To measure the AAV titer, host genome and plasmid DNA were digested with DNase I (Sigma) before releasing the viral DNA from the particles, and then the viral DNA was released by Buffer AL (QIAGEN).

    Article Title: Design and application of the transformer base editor in mammalian cells and mice.
    Article Snippet: Fusing apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like cytidine deaminase with catalytically impaired Cas proteins (e.g., nCas9 or dCas9) provides a novel gene-editing technology, base editing, that grants targeted base substitutions with high efficiency.. However, genome-wide and transcriptome-wide off-target mutations are observed in base editing, which raises safety concerns regarding therapeutic applications.. Previously, we developed a new base editing system, the transformer base editor (tBE), to induce efficient editing with no observable genome-wide or transcriptomewide off-target mutations both in mammalian cells and in mice.

    Article Title: Infectivity Assessment of Recombinant Adeno-Associated Virus and Wild-Type Adeno-Associated Virus Exposed to Various Diluents and Environmental Conditions.
    Article Snippet: Th is pa pe r h as b ee n pe er ‐re vi ew ed a nd a cc ep te d fo r p ub lic at io n, b ut h as y et to u nd er go co py ed iti ng a nd p ro of co rr ec tio n. T he fi na l p ub lis he d ve rs io n m ay d iff er fr om th is pr oo f. Infectivity assessment of recombinant adeno‐associated virus (rAAV) and wild‐type AAV (wtAAV) exposed to various diluents and environmental conditions Taro Tomono, Yukihiko Hirai, Hideto Chono, Junichi Mineno, Akiko Ishii, Masafumi Onodera, Akira Tamaoka, and Takashi Okada Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan Graduate School of Comprehensive Human Sciences, Majors in Medical Sciences, University of Tsukuba, Ibaraki, Japan Takara Bio Inc., Shiga, Japan Department of Neurology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan Department of Human Genetics, National Center for Child Health and Development, Tokyo, Japan

    Recombinant:

    Article Title: Evasion of APOBEC1-mediated Intrinsic Immunity by a Herpesvirus Uracil DNA Glycosylase Is a Determinant of Viral Encephalitis
    Article Snippet: At 10 days post-transfection, the culture supernatant was centrifuged at 10,000 ×g for 15 min at 4°C and filtrated through a 0.22-μm filter (Thermo Fisher Scientific). .. Then, recombinant AAVs were purified using an AAVpro Concentrator (TaKaRa) according to the manufacturer’s instructions, and designated AAV- ZsGreen and AAV-UGI. .. To measure the AAV titer, host genome and plasmid DNA were digested with DNase I (Sigma) before releasing the viral DNA from the particles, and then the viral DNA was released by Buffer AL (QIAGEN).

    Bioprocessing:

    Article Title: Evasion of APOBEC1-mediated Intrinsic Immunity by a Herpesvirus Uracil DNA Glycosylase Is a Determinant of Viral Encephalitis
    Article Snippet: At 10 days post-transfection, the culture supernatant was centrifuged at 10,000 ×g for 15 min at 4°C and filtrated through a 0.22-μm filter (Thermo Fisher Scientific). .. Then, recombinant AAVs were purified using an AAVpro Concentrator (TaKaRa) according to the manufacturer’s instructions, and designated AAV- ZsGreen and AAV-UGI. .. To measure the AAV titer, host genome and plasmid DNA were digested with DNase I (Sigma) before releasing the viral DNA from the particles, and then the viral DNA was released by Buffer AL (QIAGEN).

    High Throughput Screening Assay:

    Article Title: Design and application of the transformer base editor in mammalian cells and mice.
    Article Snippet: Fusing apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like cytidine deaminase with catalytically impaired Cas proteins (e.g., nCas9 or dCas9) provides a novel gene-editing technology, base editing, that grants targeted base substitutions with high efficiency.. However, genome-wide and transcriptome-wide off-target mutations are observed in base editing, which raises safety concerns regarding therapeutic applications.. Previously, we developed a new base editing system, the transformer base editor (tBE), to induce efficient editing with no observable genome-wide or transcriptomewide off-target mutations both in mammalian cells and in mice.

    Cell Culture:

    Article Title: Infectivity Assessment of Recombinant Adeno-Associated Virus and Wild-Type Adeno-Associated Virus Exposed to Various Diluents and Environmental Conditions.
    Article Snippet: Th is pa pe r h as b ee n pe er ‐re vi ew ed a nd a cc ep te d fo r p ub lic at io n, b ut h as y et to u nd er go co py ed iti ng a nd p ro of co rr ec tio n. T he fi na l p ub lis he d ve rs io n m ay d iff er fr om th is pr oo f. Infectivity assessment of recombinant adeno‐associated virus (rAAV) and wild‐type AAV (wtAAV) exposed to various diluents and environmental conditions Taro Tomono, Yukihiko Hirai, Hideto Chono, Junichi Mineno, Akiko Ishii, Masafumi Onodera, Akira Tamaoka, and Takashi Okada Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan Graduate School of Comprehensive Human Sciences, Majors in Medical Sciences, University of Tsukuba, Ibaraki, Japan Takara Bio Inc., Shiga, Japan Department of Neurology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan Department of Human Genetics, National Center for Child Health and Development, Tokyo, Japan

    Transfection:

    Article Title: Infectivity Assessment of Recombinant Adeno-Associated Virus and Wild-Type Adeno-Associated Virus Exposed to Various Diluents and Environmental Conditions.
    Article Snippet: Th is pa pe r h as b ee n pe er ‐re vi ew ed a nd a cc ep te d fo r p ub lic at io n, b ut h as y et to u nd er go co py ed iti ng a nd p ro of co rr ec tio n. T he fi na l p ub lis he d ve rs io n m ay d iff er fr om th is pr oo f. Infectivity assessment of recombinant adeno‐associated virus (rAAV) and wild‐type AAV (wtAAV) exposed to various diluents and environmental conditions Taro Tomono, Yukihiko Hirai, Hideto Chono, Junichi Mineno, Akiko Ishii, Masafumi Onodera, Akira Tamaoka, and Takashi Okada Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan Graduate School of Comprehensive Human Sciences, Majors in Medical Sciences, University of Tsukuba, Ibaraki, Japan Takara Bio Inc., Shiga, Japan Department of Neurology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan Department of Human Genetics, National Center for Child Health and Development, Tokyo, Japan

    other:

    Article Title: Herpes simplex virus 1 evades APOBEC1-mediated immunity via its uracil-DNA glycosylase in mice.
    Article Snippet: Herpes simplex virus 1 (HSV-1) is the most common cause of viral encephalitis, which can be lethal or result in severe neurological defects despite antiviral therapy.. The apolipoprotein B messenger-RNA editing enzyme, catalytic polypeptide-like (APOBEC) group of proteins can act as viral restriction factors.. How HSV-1 evades this intrinsic immune mechanism is unclear.



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